| NAME | PLAYER NUMBER | DEBUT |
| Sarah Denholm | 1 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Alex Stewart | 2 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Cieron Bell | 3 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Gemma Bell | 4 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Poppy Fletcher | 5 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Merryn Gunderson | 6 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Nicola Haynes | 7 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Katie Lindsay | 8 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Natasha Logan | 9 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Nicole Marlow | 10 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Jenny Maxwell | 11 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Fiona McIntosh | 12 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Briar McNamara | 13 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Rachel Philipps | 14 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Hannah Walker | 15 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Leia Brebner-Holden | 16 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Panashe Muzambe | 17 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Molly Poolman | 18 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Millie Whitehouse | 19 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Freya Walker | 20 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Hannah Chrisp | 21 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Hannah Ramsay | 22 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Adelle Ferrie | 23 | vs Glasgow Warriors, 30 December 2023, Hive Stadium |
| Emma Orr | 24 | vs Wolfhounds, 6 January 2024, Hive Stadium |
| Nicole Flynn | 25 | vs Wolfhounds, 6 January 2024, Hive Stadium |
| Caity Mattinson | 26 | vs Brython Thunder, 14 January 2024, Parc y Scarlets |
| Aila Ronald | 27 | vs Brython Thunder, 14 January 2024, Parc y Scarlets |
| Sophie Murphy | 28 | vs Brython Thunder, 14 January 2024, Parc y Scarlets |
| Lisa Thomson | 29 | vs Gwalia Lightning, 28 January 2024, Cardiff Arms Park |
| Samaanther Taganekurukuru | 30 | vs Clovers, 3 February 2024, Hive Stadium |
| Rhona Lloyd | 31 | vs Wolfhounds, 17 February 2024, Hive Stadium |
| Zoe Turner | 32 | vs Wolfhounds, 17 February 2024, Hive Stadium |
| Lucy Kidd | 33 | vs Clovers, 3 March 2024, Parc y Scarlets |
| Alison Wilson | 34 | vs Clovers, 3 March 2024, Parc y Scarlets |
| Rhea Clarke | 35 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Lucy MacRae | 36 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Holly McIntyre | 37 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Lucia Scott | 38 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Shona Campbell | 39 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Talei Tawake | 40 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Lucy Christie | 41 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Karis Craig | 42 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Hannah McMahon | 43 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Giselle Chicot | 44 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Faye Sutherland | 45 | vs Glasgow Warriors, 20 December 2024, Hive Stadium |
| Lisa Brown | 46 | vs Brython Thunder, 4 January 2025, Hive Stadium |
| Charlotte Fosbeary | 47 | vs Brython Thunder, 4 January 2025, Hive Stadium |
| Kaylee Fraser | 48 | vs Brython Thunder, 4 January 2025, Hive Stadium |
| Pip Benson | 49 | vs Brython Thunder, 4 January 2025, Hive Stadium |
| Lauryn Walter | 50 | vs Clovers, 11 January 2025, Virgin Media Park |
| Megan Riach | 51 | vs Clovers, 11 January 2025, Virgin Media Park |
| Chloe Brown | 52 | vs Gwalia Lightning, 26 January 2025, Hive Stadium |
| Ami Conchie | 53 | vs Brython Thunder, 8 February 2025, Parc y Scarlets |
| Rachel Law | 54 | vs Glasgow Warriors, 15 February 2025, Scotstoun Stadium |
| Emily Love | 55 | vs Glasgow Warriors, 15 February 2025, Scotstoun Stadium |
| Charlotte Russell | 56 | vs Glasgow Warriors, 15 February 2025, Scotstoun Stadium |
| Cat Moody | 57 | vs Wolfhounds, 22 February 2025, Hive Stadium |
| Rowan McPherson | 58 | vs Wolfhounds, 8 March 2025, Energia Park |
| Millie Capaldi | 59 | vs Wolfhounds, 8 March 2025, Energia Park |
| April McKenzie | 60 | vs Wolfhounds, 8 March 2025, Energia Park |
